Abstract: As a new mode of industrial space， the “industrial upstairs” concept is explored in dense urban agglomerations. This study analyzes the effects of peak acceleration on an adjacent high-precision laboratory and an adjacent biomedical laboratory caused by vehicle dynamic loads in horizontal freight corridors. The results showed that the peak acceleration increased with the number of vehicle floors. The peak acceleration generated by continuous vehicle movement was significantly greater than that from a single vehicle. Furthermore， the peak acceleration at each measurement point decreased as the distance from the vibration source increased. A vibration isolation trench was constructed between the horizontal freight corridor and the high-precision laboratory to lengthen the propagation path of vibration waves and reduce the peak acceleration at the measurement points. Compared to the scenario without a trench， the reduction in peak acceleration was insignificant when the trench depth was within 7.5 meters. However， when the trench depth increasesd from 7.5 meters to 12.5 meters， the peak acceleration decreased markedly， demonstrating an obvious vibration reduction effect. It is necessary to analyze the structural dynamic response at different trench depths to determine reasonable technical parameters for the vibration isolation trench in engineering design.