The fatigue tests of flexural tension-compression under constant amplitude cyclic loading have been carried out on pre-stressed concrete cantilever plates against the fatigue state of flexural tension-compression in pre-stressed concrete noise barrier used in high-speed railway.The results of the tests have been presented in the relationship of S-N and compared with those obtained by other researchers.It is also analyzed that tensile fatigue strength of the concrete and the loss rate of effective pre-stress under cyclic loading based on the results of the experiment.The results show that the fatigue capacity of concrete under flexural tension-compression is better than that under axial tension-compression.The loss rate of effective pre-stress under cyclic loading ranged from 3%~6%.The results of investigation furnish the evidence for the design and analysis of fatigue crack resistance for pre-stressed concrete structure under cyclic loading.