Fire-resistant performance of GluBam wall is one of most important factors for the modern bamboo structural buildings. However,the research in this field is still in the blank. In this paper,a fire-resistant test of the full-scale GluBam wall was conducted under ISO standard temperature curve. The temperature characteristics,fireresistant influence factors and failure mode were gained after the test. Temperature curves of each measuring point, temperature distribution and fire-resistant limit were also recorded. Using fire dynamics simulation software FDS to simulate the gluing bamboo wall structure under ISO 834 standard fire conditions. The research results showed that the unexposed surface highest temperature did not exceed 90 ℃ and the temperature distribution was closely related to the measuring point location. The failure mode was E class. The duration of fire resistance was more than 60 min, which could well meet the requirements of code. The fire dynamics simulator,FDS,could be well used to simulate the temperature of GluBam structural wall under the test conditions. The temperature rising trend and distribution were basically consistent between the simulated results and measured ones. The factors which influence fire-resistant performance of GluBam wall need to be researched further.