The fatigue performance of high strength steel was studied so as to discuss the relevant steel fatigue design provisions of the code for design of steel structures and help to promote the application of high strength steel in civil engineering. which provided data reference for following up research. A number of axial tensile fatigue tests by conventional test methods using Q460D plate specimens were conducted and the S-N curves attached to the fatigue performance were drawn. The test results showed that the fatigue performance of these Q460D steel specimens was very sensitive to the fatigue load and the data discreteness was considerable. The fatigue strength of Q460D under 2 million times loading was greater than that calculate by Code for Design of Steel Structuares(GB 500172003).