Abstract: The prestressed space cable-stayed suspension structure is a special structural form in the long-span space structure. It is mainly composed of diagonal load-bearing cables, central steel structure and lower stabilizer cables. The cable-stayed houseboat structure of the comprehensive building of Ocean University of China is a typical application of this structure. It mainly includes roof grid, houseboat steel members, load-bearing cables and stabilizer cables. Based on this engineering example, the paper proposed the construction method of the cable-stayed suspension structure. Firstly, the central steel structure was lifted to the height by diagonally pulling the load-bearing cable. Then the counterweight tooling cable was installed and tensioned. Then the daylighting roof and the stabilizer cable were installed in sequence and the counterweight tooling cable was removed. In the process, it was one of the keys to keep the central steel structure at the design elevation by adjusting the cable force of the counterweight tooling. According to the structural characteristics and construction methods, the force-finding analysis of the load-bearing cables and counterweight tooling cables in key construction conditions and the analysis of typical conditions in the construction process were conducted. The change laws of structural response changes were revealed and the predetermined goals of the key conditions were reached.