Abstract: The urban underground pipe gallery often produces adverse effects on the deformation and uneven settlement of upper ground building after construction. Under the horizontal earthquake action after the construction of the underground pipe gallery, the adverse effects will be magnified, even beyond the normal use scope of the upper structure. Based on a practical project, the seismic response of the building structure before and after the construction of the underground pipe gallery was analyzed by the finite element method based on the nonlinear time history analysis proposed by Standard for Seismic Design of Underground Structures (GB/T 51336-2018). The seismic response difference of the structure before and after the construction of the pipe gallery was obtained, and the reasons for the difference were analyzed by using the calculation results. By simulating the design and construction measures of grouting reinforcement around the pipe gallery and adjusting the structural stiffness of the pipe gallery, the seismic response of the superstructure was obtained, and the effective control measures for improving the construction of the pipe gallery under the existing high-rise building were obtained. After the completion of the actual project, the soil samples before and after grouting reinforcement were extracted for indoor dynamic triaxial test. The test results could further support the improvement effect of grouting on the dynamic characteristics of foundation soil and the control effect on the seismic response of upper structure.