Abstract: The cultural venues has the characteristics of complex space and system, high requirements for reliability and comfort of operation and maintenance. The traditional passive operation and maintenance mode of "problem-solving" is difficult to meet the requiements of operation, so there is a strong demand for intelligent operation and maintenance system. The practical experience of intelligent operation and maintenance system based on digital twins after the opening of Shanghai Astronomy Museum was snmmarized, including implementation methods, application values, existing problems and countermeasures, so as to provide a reference for the implementation of intelligent operation and maintenance system in subsequent science and technology exhibition halls and other buildings. The application practice showed that although it was difficult to review and improve the BIM model, repair the building intelligent systems and merge their data after the opening, with the strong support of the owner, the construction of building intelligent operation and maintenance system could still be completed within the building maintenance time, and effectively improve the level and efficiency of building intelligent operation and maintenance, as well as the reliability.