一种高效碳酸盐矿化菌的筛选及砂土固化试验研究

李湘; 夏百惠; 宋天顺; 谢婧婧

doi:10.13204/j.gyjzG22061309

一种高效碳酸盐矿化菌的筛选及砂土固化试验研究

doi: 10.13204/j.gyjzG22061309

1. 南京工业大学生物与制药工程学院, 南京 211816;
2. 南京工业大学交通运输工程学院, 南京 211816

基金项目:

国家重点研发计划项目(2021YFA0910400)。

详细信息

作者简介:
李湘,女,1997年出生,硕士研究生,lixiang9708@163.com。

通讯作者:
宋天顺,男,1981年出生,博士,副研究员,tshsong@njtech.edu.cn。

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出版历程
- 收稿日期: 2022-06-13

Screening of a Highly Effective Carbonate Mineralized Bacterium and Its Cementation in Sand Soil

1. College of Life Science and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China;
2. College of Transportation Engineering, Nanjing Tech University, Nanjing 211816, China

摘要

摘要: 从盐湖附近分离筛选出多株菌,通过诱导碳酸盐沉积能力测定,最终选出诱导碳酸盐沉积能力最强的菌株X-NM1,经16S rDNA分析鉴定为表皮葡萄球菌。其诱导生成的碳酸钙沉积量为16.8 g/L,脲酶活性为3.35 mM/min,其矿化产物为类球状方解石晶体。进一步将该X-NM1通过砂土固化试验,考虑不同灌浆次数对砂柱固化的效果。结果表明:随着灌浆循环次数的增加,砂柱试样的渗透系数、孔隙率不断下降,而干密度、孔隙填充率、单轴抗压强度及碳酸钙含量逐步增大。3次灌浆后,得到的砂柱样品外观结构完整,干密度为1.99 g/cm³、渗透系数为3.60×10^-4 cm/s、孔隙率为26.35%、孔隙填充率为15.42%、单轴抗压强度为2.52 MPa、CaCO₃含量达24.5%,表明X-NM1具有优良的诱导碳酸钙沉积能力。
- 碳酸盐矿化菌 /
- 表皮葡萄球菌 /
- 碳酸钙 /
- 砂土固化
Abstract: Several varieties of bacteria were isolated and screened from the vicinity of the Salt Lake. The X-NM1 with the strongest capability to induce carbonate deposition was finally selected. It was identified as Staphylococcus epidermidis by 16S rDNA analysis. The induced calcium carbonate deposition was 16.8 g/L, the urease activity was 3.353 mM/min, and the mineralization product was spherical calcite crystals. The X-NM1 was further tested by sand cementation, considering the effect of grouting cycles on sand column solidification. The results showed that with the increase of grouting cycles, the permeability coefficient and porosity of sand columns decreased, while the dry density, pore filling ratio, uniaxial compressive strength and calcium carbonate content increased gradually. After three cycles of grouting, the appearance structure of sand columns was intact, the dry density, the permeability coefficient, the porosity, the pore filling ratio, the uniaxial compressive strength, and the CaCO₃ content was 1.99 g/cm³, 1.51×10^-3 cm/s, 26.35%, 15.42%, 2.52 MPa, and 24.5% respectively. The results showed that X-NM1 was of excellent capability to induce calcium carbonate deposition.
- carbonate mineralizing bacteria /
- Staphylococcus epidermidis /
- CaCO₃ /
- sand cementation

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