Screening of a Highly Effective Carbonate Mineralized Bacterium and Its Cementation in Sand Soil
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摘要: 从盐湖附近分离筛选出多株菌,通过诱导碳酸盐沉积能力测定,最终选出诱导碳酸盐沉积能力最强的菌株X-NM1,经16S rDNA分析鉴定为表皮葡萄球菌。其诱导生成的碳酸钙沉积量为16.8 g/L,脲酶活性为3.35 mM/min,其矿化产物为类球状方解石晶体。进一步将该X-NM1通过砂土固化试验,考虑不同灌浆次数对砂柱固化的效果。结果表明:随着灌浆循环次数的增加,砂柱试样的渗透系数、孔隙率不断下降,而干密度、孔隙填充率、单轴抗压强度及碳酸钙含量逐步增大。3次灌浆后,得到的砂柱样品外观结构完整,干密度为1.99 g/cm3、渗透系数为3.60×10-4 cm/s、孔隙率为26.35%、孔隙填充率为15.42%、单轴抗压强度为2.52 MPa、CaCO3含量达24.5%,表明X-NM1具有优良的诱导碳酸钙沉积能力。Abstract: Several varieties of bacteria were isolated and screened from the vicinity of the Salt Lake. The X-NM1 with the strongest capability to induce carbonate deposition was finally selected. It was identified as Staphylococcus epidermidis by 16S rDNA analysis. The induced calcium carbonate deposition was 16.8 g/L, the urease activity was 3.353 mM/min, and the mineralization product was spherical calcite crystals. The X-NM1 was further tested by sand cementation, considering the effect of grouting cycles on sand column solidification. The results showed that with the increase of grouting cycles, the permeability coefficient and porosity of sand columns decreased, while the dry density, pore filling ratio, uniaxial compressive strength and calcium carbonate content increased gradually. After three cycles of grouting, the appearance structure of sand columns was intact, the dry density, the permeability coefficient, the porosity, the pore filling ratio, the uniaxial compressive strength, and the CaCO3 content was 1.99 g/cm3, 1.51×10-3 cm/s, 26.35%, 15.42%, 2.52 MPa, and 24.5% respectively. The results showed that X-NM1 was of excellent capability to induce calcium carbonate deposition.
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Key words:
- carbonate mineralizing bacteria /
- Staphylococcus epidermidis /
- CaCO3 /
- sand cementation
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[1] 骆晓伟. 基于微生物诱导碳酸钙沉淀技术(MICP)的砂土固化试验研究[D]. 南京:南京大学, 2018. [2] 钱春香, 王安辉, 王欣. 微生物灌浆加固土体研究进展[J]. 岩土力学, 2015, 36(6):1537-1548. [3] DEJONG J T, MORTENSEN B M, MARTINEZ B C. Biomediated soil improvement[J]. Ecological Engineering, 2010, 36(2):197-210. [4] HARKES M P, VAN PAASSEN L A, BOOSTER J L, et al. Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement[J]. Ecological Engineering, 2010, 36(2):112-117. [5] MITCHELL J K, SANTAMARINA J C. Biological considerations in geotechnical engineering[J]. Journal of Geotechnical and Geoenvironmental Engineering, 2005, 131(10):1222-1233. [6] 季斌, 陈威, 樊杰, 等. 产脲酶微生物诱导钙沉淀及其工程应用研究进展[J].南京大学学报(自然科学版), 2017, 53(1):191-198. [7] 张茜, 叶为民, 刘樟荣, 等. 基于生物诱导碳酸钙沉淀的土体固化研究进展[J]. 岩土力学, 2022, 43(2):345-357. [8] CHU J, IVANOV V, STABNIKOV V, et al. Microbial method for construction of aquaculture pond in sand[J]. Géotechnique, 2013, 63(10):871-875. [9] SEKI K, MIYAZAKI T, NAKANO M. Effects of microorganisms on hydraulic conductivity decrease in infiltration[J]. European Journal of Soil Science, 1998, 49(2):231-236. [10] PHILLIPS A J, LAUCHNOR E, ELDRING J, et al. Potential CO2 leakage reduction through biofilm-induced calcium carbonate precipitation[J]. Environmental Science & Technology, 2013, 47(1):142-149. [11] 刘汉龙, 肖鹏, 肖杨, 等. 微生物岩土技术及其应用研究新进展[J]. 土木与环境工程学报(中英文), 2019, 41(1):1-14. [12] HAMMES F, SEKA A, DE KNIJF S, et al. A novel approach to calcium removal from calcium-rich industrial wastewater[J]. Water Research, 2003, 37(3):699-704. [13] 孙莺. 中国不同地区尿素分解菌的分离筛选及其诱导碳酸钙沉积能力的比较研究[D]. 兰州:兰州大学, 2017. [14] KANG C H, HAN S H, SHIN Y J, et al. Bioremediation of Cd by microbially induced calcite precipitation[J]. Applied Biochemistry and Biotechnology, 2014, 172(4):1929-1937. [15] DHAMI N K, REDDY M S, MUKHERJEE A, et al. Biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites[J]. World Journal of Microbiology and Biotechnology, 2013, 23(5):707-714. [16] HAMMES F, BOON N, VILLIERS J D, et al. Strain-specific ureolytic microbial calcium carbonate precipitation[J]. Applied & Environmental Microbiology, 2003, 69(8):4901-4909. [17] 张越. 微生物用于砂土胶凝和混凝土裂缝修复的试验研究[D]. 北京:清华大学, 2014. [18] BUCHANAN R E, GIBBONS N E. 伯杰氏系统细菌学手册[M].8版.北京:科学出版社,1984. [19] WHIFFIN V S. Microbial CaCO3 precipitation for the production of biocement[D]. Perth:Murdoch University, 2004. [20] 斯日古楞. 砂土基微生物诱导矿化材料微细观力学特性研究[D].呼和浩特:内蒙古工业大学, 2021. [21] 赵阿龙. 微生物固化石油污染砂土的力学特性研究[D]. 镇江:江苏大学, 2021. [22] 徐宏殷. 微生物花管注浆砂土固化试验和仿真模拟研究[D]. 天津:天津大学, 2020. [23] 史冠宇. 脲酶沉积碳酸钙在土中发挥固化效用的试验研究[D]. 呼和浩特:内蒙古工业大学, 2020. [24] 张振远. 碳酸盐矿化菌的分离筛选及生物注浆实验研究[D]. 衡阳:南华大学, 2014. [25] 赵茜. 微生物诱导碳酸钙沉淀(MICP)固化土壤实验研究[D]. 北京:中国地质大学, 2014.
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