Abstract: With the continuous expansion of the scale of the subway, a long span, more spacious space, more comfortable ride feeling has become the requirements of the people. Long-span column-free station can effectively expand the use space of station structure and improve passengers’ transfer experience by canceling the pier columns on platform floor. However, the long-span structure without columns also brings great difficulties to the structural design of the station. Steel-concrete composite structure has excellent mechanical properties and convenient construction, and can obtain good comprehensive benefits in solving the problems of long-span structures. Relying on the South Third Ring Station project of Xuzhou Rail Transit Line 3, the steel-concrete composite structure scheme of transfer node station was proposed. MIDAS Gen was used to establish an overall model for analysis, and the mechanical properties of the structure and the stress states of key components were studied. The results showed that the steel-concrete composite structure scheme could significantly reduce the weight of the structure, save the building space, and exhibited the advantages of convenient and fast construction. The results of modeling analysis showed that the long-span transfer beam was in the state of bending, shear and torsion, which was the key point and difficulty of design. Finally, some design suggestions were put forward for the application of steel-concrete composite structure scheme in long-span column-free station structure, which could provide a reference for similar projects in the future.