大豆脲酶对Zn<sup>2+</sup>污染土的修复试验研究

边汉亮; 张旭钢; 韩一; 李贝贝; 张建伟

doi:10.13204/j.gyjzG20110216

大豆脲酶对Zn²⁺污染土的修复试验研究

doi: 10.13204/j.gyjzG20110216

边汉亮^1,2,3,
张旭钢³,
韩一³,
李贝贝³,
张建伟^1,2,3, ,

1. 开封市特殊土改性与修复工程技术研究中心, 河南开封 475004;
2. 河南省轨道交通智能建造工程技术中心, 河南开封 475004;
3. 河南大学土木建筑学院, 河南开封 475004

基金项目:

河南省科技发展计划项目(202102310575)

河南省高等学校青年骨干老师培养计划(2019GGJS041)。

河南省科技厅科技攻关重点项目(222102320355)

详细信息

作者简介:
边汉亮,男,1981年出生,博士,讲师,bian2000@126.com。

通讯作者:
张建伟,1981年出生,博士,教授,硕士生导师,zjw101_0@163.com。

计量
- 文章访问数: 131
- HTML全文浏览量: 17
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2020-11-02

Remediation Tests of Zn²⁺ Contaminated Soil by Soybean Urease

BIAN Hanliang^1,2,3,
ZHANG Xugang³,
HAN Yi³,
LI Beibei³,
ZHANG Jianwei^{1,2,3
, ,}

1. Special Soil Modification and Restoration Engineering Technology Research Center of Kaifeng, Kaifeng 475004, China;
2. Henan Provincial Research Center of Engineering on Intelligent Construction of Rail Transit, Kaifeng 475004, China;
3. School of Civil Engineering and Architecture of Henan University, Kaifeng 475004, China

摘要

摘要: 为了研究植物脲酶诱导碳酸钙沉淀技术(EICP)对Zn²⁺污染土的处理效果,从大豆中提取了丰富的脲酶,在底物诱导下实现对重金属污染土中Zn²⁺的矿化处理。通过Tessier五步连续提取法对修复前、后污染土中不同形态Zn²⁺进行提取检测,并对不同修复次数的土体进行无侧限抗压强度试验。结果表明:所提取的大豆脲酶能有效催化尿素水解生成碳酸根离子,使其在底物诱导下生成碳酸盐沉淀并矿化Zn²⁺;EICP技术能有效降低污染砂土中Zn²⁺的含量,使其以碳酸盐的形式固定封存;随着修复次数的增加,砂土的无侧限抗压强度从碎散状0 MPa增加至0.44 MPa。EICP技术为重金属Zn²⁺污染土修复提供了新的选择。
- 大豆脲酶 /
- 脲酶诱导碳酸盐沉淀 /
- 重金属Zn²⁺ /
- 污染土
Abstract: In order to study the treatment effect of Enzyme Induced Calcium Carbonate Precipitation (EICP) on Zn²⁺ contaminated soil, abundant urease was extracted from soybean, mineralization of heavy metal Zn²⁺ with substrate induction was implemented. The different forms of Zn²⁺ in contaminated sand before and after remediation were extracted and detected by Tessier’s five-step continuous extraction method, and the mechanical properties of different remediation times were compared through unconfined compressive strength tests. Results showed that the extracted urease had a good activity and it could effectively catalyzed the hydrolysis of urea to form carbonate ions, which could form carbonate precipitation and mineralize Zn²⁺ in the induction of substrate. EICP technology could effectively reduce the content of Zn²⁺ in contaminated sand, and fix it in the form of carbonate, moreover, the unconfined compression strength of the repaired sand increased from 0 to 0.44 MPa after repaired 3 times. EICP technology provided a new choice for the remediation of heavy metal Zn²⁺ contaminated soil.
- soybean urease /
- urease induction of carbonate precipitation /
- heavy metal Zn²⁺ /
- contaminated soil

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参考文献(17)

[1]	邓代莉,石清清,薛圣炀,等.外源铅污染对紫色土中微生物酶活性的影响研究[J].环境污染与防治,2018,40(10):1095-1100.
[2]	刘松玉.污染场地测试评价与处理技术[J].岩土工程学报,2018,40(1):1-37.
[3]	王茂林,吴世军,杨永强,等.微生物诱导碳酸盐沉淀及其在固定重金属领域的应用进展[J].环境科学研究,2018,31(2):206-214.
[4]	ACHAL V, PAN X, ZHANG D, et al. Bioremediation of Pb-contaminated soil based on microbially induced calcite precipitation[J]. Journal of Microbiol Biotechnol, 2012, 22(2):244-247.
[5]	TESSIER A, CAMPBELL P G C, BISSON M. Sequential extraction procedure for the speciation of particulate trace metals[J]. Analytical Chemistry, 1979,51(7):844-851.
[6]	许朝阳,柏庭春,黄建璋,等.铁细菌修复锌污染土壤的试验研究[J].工业建筑,2016,46(6):90-93 ,132.
[7]	王新花,赵晨曦,潘响亮.基于微生物诱导碳酸钙沉淀(MICP)的铅污染生物修复[J].地球与环境,2015,43(1):80-85.
[8]	DILRUKSHI R A N, KAWASAKI S. Effect of plant-Derived urease-induced carbonate formation on the strength enhancement of sandy soil[G]//Ecological Wisdom Inspired Restoration Engineering. Singapore:Springer, 2019:93-108.
[9]	KANG C H, SO J S. Heavy metal and antibiotic resistance of ureolytic bacteria and their immobilization of heavy metals[J]. Ecological Engineering, 2016, 97:304-312.
[10]	NEUPANE D, YASUHARA H, KINOSHITA N, et al. Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique[J]. Journal of Geotechnical and Geoenvironmental Engineering, 2013, 139(12):2201-2211.
[11]	吴林玉,缪林昌,孙潇昊,等.植物源脲酶诱导碳酸钙固化砂土试验研究[J].岩土工程学报,2020,45(4):714-720.
[12]	张铁军,施圆圆,孔令漪,等.黄豆豆渣中脲酶的提取精制及其影响因素研究[J].生物技术进展,2017,7(3):253-257.
[13]	崔有宏,罗侃,吴育凌,等.黄豆脲酶的提取与性质研究[J].甘肃科学学报,2000(1):62-66.
[14]	周东凯,刘莹,马学良,等.大豆脲酶的提取及其影响因素研究[J].大豆科学,2008(4):704-707.
[15]	邹菁,喻德忠,杨先进,等.黄豆脲酶的提取及其在缓释肥料中尿素态氮测定中的应用[J].分析科学学报,2004(2):178-180.
[16]	杨丰,何稼,亓永帅,等.大豆脲酶基本特性与粉质砂土的固化研究[J].河南科学,2019,37(1):112-118.
[17]	WHIFFIN V S. Microbial CaCO₃ precipitation for the production of bio-cement[D]. Perth:Murdoch University, 2004.