Abstract: At present, there is still a lack of vibration testing and comfort evaluation method for the composite floor slabs of the giant steel frame with suspended structure systems at home and abroad. In order to reveal the vibration characteristics and comfort of the composite floor slab of the giant steel frame with suspended structure system under the excitation of human-induced loads, on-site vibration measurement and analysis were carried out based on the research building of the Quantum Academy of the Chinese Academy of Sciences. The frequency distribution of the floor slab was obtained through the dynamic characteristic test; the human-induced load excitation and rhythm jump excitation were applied to the floor slab, focusing on the analysis of the influence of the main factors such as step frequency, pedestrian number, and walking route on the floor vibration comfort of the new suspended structural system. The test results showed that the peak acceleration of the floor slab gradually increased with the increase of the stride frequency and the number of people walking; walking along the width of the floor slab would cause a significant vibration response; rhythmic jump excitation has a great impact on floor comfort, and excessive jump excitation should be avoided when using it. Combining with domestic and foreign codes, this floor was evaluated and analyzed for vibration comfort. The research results showed that the main vibration frequency and vibration intensity could be used to evaluate the vibration comfort of the floor slab of the new structure, which meant that the main vibration frequency should not be less than 3 Hz, and the vibration intensity should not be greater than 0.015 m/s²