Abstract: The standing seam metal roof system has the advantages of light weight, large spans, high strength and good waterproof performances. Therefore, it was increasingly used in airports, train stations and stadiums. However, due to the lack of the corresponding design and construction specifications, the disadvantage of the insufficient wind uplift resistance was gradually reflected. Taking the repair and reinforcement project of metal roofs for terminal T2 at Changbei Airport as the research background, the wind uplift resistance of metal roof panels with standing seam systems was tested and evaluated by full-scale tests. The reasonable reinforcement measures of the metal roof with standing seam systems were determined by analyzing failure modes of wind uplift and variation rules of wind uplift resistance for the metal roof panels with standing seam systems in different reinforcement modes. The test results showed that the clamps could effectively improve the wind uplift resistance of HXY-400 standing seam metal roof panels, and its wind uplift resistence was 4.18 times higher than that of the roof panels without clamps. In addition, purlin spacing also had a direct impact on the wind uplift resistance of standing seam metal roof panels.