Abstract: The floor vibration responses induced human of the new type assembly prefabricated steel concrete composite slab used in the building named Xiao Tiancheng were measured and computed，it was obtained the peak acceleration due to vertical vibrations of the new type floor under six kinds of load cases，i． e single person walking， five people walking，single person running，five people running，single person jumping and five people jumping． The analysis results were in good agreement with the test results． Loading modes，walking route and load frequency which influenced the peak acceleration were analyzed． The results indicated that the jump was the most unfavorable loading mode for floor vibration; the more people，the greater the floor vibration; high order harmonic component of pedestrian load could cause the resonance of the floor; the new type of floor could meet the comfort requirement．