Abstract: The bacteria Sporosarcina pasteurii ( S． p) solution and the urease of S． p was added into the cement mortar． The urea-calcium acetate solution，bacterial culture medium and water were used to cure specimens． The uniaxial compressive test，the split tensile strength test and freeze-thaw cycling test were carried out to study the changes of their strength and durability ． The mercury intrusion porosimeter was used to understand the microstructure of microbial cement mortar． The results showed that the microbial cement mortar had an increase in strength，the highest compressive strength increased by 47. 22%，the highest tensile strength increased by 60. 39%． In addition， the microbial cement mortar specimen cured in water had an excellent performance in freeze-thaw cycle test．