Abstract: Microbial-induced clogging is a method of leakage repair，with which the composition of strains in a soil can be changed through induction of microbes，thus changing the produced metabolites，which can finally change the permeability of the soil and stop the seepages of grounds． This technology has been successfully applied to the leakage repair of some underground seepage control projects，such as tunnel，storage facilities． But the technology still belongs to an empirical technique，the mechanism of the series of chemical biology reactions and the relationship between microbiology and soil need to be studied． In the study，nutrient solutions were injected into sand columns with leakage and the mechanism of clogging，the number of microorganisms and change in microbial communities in different locations of individual sand columns were analyzed．