Abstract: Based on current specifications timber materials，such as Wood Physical and Mechanical Properties Test Methods and Standard Test Methods for Small Clear Specimens of Timber ( ASTM D 143 －94) ，it was conducted shear tests of glue laminated bamboo ( GluBam) ，with different loading configurations related to the main fiber orientation． It was obtained the average value of in-plane and out-of-plane shear strengths and found out by statistical analysis of the shear test data that: Weibull distribution could be used to describe the distribution of the out-of-plane shear strength，normal distribution could better meet in-plane shear strength． The results showed that fiber arrangement and loading have great influence on shear strength． By comparing with several other common building materials，GluBam could meet the shear strength requirements of structure．