Abstract: If the dimension of rectangular openings designed suitably in practical projects，pipes and wires will not only expediently pass through the beam web to reduce the story height of a building and increase economic benefits，but also move the plastic hinge far away from the connection. In this way，it can avoid the connection failure before the members，and ensures the structural integrity and make it no collapse. The dimension of openings and the distance between the opening and the column surface are determined by the restriction requirement for beam web by flange，the shear-bearing requirements of web，the ratio of the moment at beam end to the moment at reduced section and preventing compressive plate local buckling. While with openings on web，the element stiffness matrix of frame element and the internal force and displacements of structure will be affected out of question. Therefore，it is necessary to research the element stiffness matrix of frame element. In order to decrease the amount of calculation，the beam is handled as a link element. Virtual work theory is used to derive the element stiffness matrix of frame element with openings on web，and compared with the result of finite analysis method. It shows a satisfactory agreement between calculated and finite analysis results.